侵染葫芦的黄瓜绿斑驳花叶病毒广西分离物分子鉴定

Molecular identification of an isolate of Cucumber green mottle mosaic virus infecting bottle gourd in Guangxi

从广西南宁市郊温室大棚中的葫芦[Lagenaria siceraria(Molina)Stand.]上采集到一个表现脉绿、花叶症状的病毒样品,ELISA检测表明,该样品与黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic virus,CGMMV)有密切的血清学关系,利用RT-PCR方法从样品中扩增获得约500bp的DNA片段,序列分析表明,该片段是CGMMV的外壳蛋白基因,暂将该病毒分离物定名为GX-BG。外壳蛋白基因核苷酸序列系统进化树分析表明,已报道的CGMMV主要分为3大群体,GX-BG与中国辽宁分离物(CGMMV-LN)分别属于不同的群体。

A virus isolate from bottle gourd （GX-BG） with green veins and mosaic symptoms was collected in greenhouses in the suburb of Nanning. ELISA tests indicated that GX-BG had close serological relationships with Cucumber green mottle mosaic virus （CGMMV）. By using specific primers designed based on the coat protein gene of CGMMV, a 500-bp segment was obtained by RT-PCR. A BLAST search confirmed that it was an isolate of CGMMV. The previously reported CGM- MVs could be classified as three main groups according to phylogenetic analysis based on the coat protein gene nucleotide sequences of CGMMV. GX-BG and CGMMV-LN belonged to different groups.