环孢素A对实验大鼠肺纤维化的干预作用

Effects of cyclosporin A on pulmonary fibrosis in rats

目的研究环孢素A(CsA)对大鼠肺纤维化的干预作用。方法平阳霉素(BLM)气管内注入建立大鼠肺纤维化模型,观察CsA对支气管肺泡灌洗液(BALF)中细胞成分和转化生长因子-β(TGF-β)的含量、肺组织学变化,应用SABC法检测肺组织中纤维连接蛋白(FN)和血小板衍化生长因子(PDGF)表达的作用。结果应用BLM后第7天即有严重肺泡炎性改变,第28天有严重的肺纤维化,而CsA组,早期只有轻度炎性改变,BALF中细胞计数及TGF-β含量较BLM组低(P<0.05),FN及PDGF表达显著弱于BLM组(P<0.01)。结论CsA可能通过抑制FN、PDGF的表达及TGF-β的生成干预BLM致肺纤维化。

Objective To study the effects of cyclosporin A on pulmonary fibrosis induced by bleomycin in rats. Methods Forty-five Wistar rats were divided into three groups in random： saline group, untreated model group and cyclosporin A-treated group. Bronchoalveolar lavage was performed in the rats for the enumeration of cells and the concentrations of translation growth factor-β（TGF-β）. Lung tissues of tested animals were pathologically observed and immunohistochemically stained. Results On the 7th day after bleomycin instillation, serious alveolitis presented. On the 28th day, the serious pulmonary fibrosis formed. In contrast to the Neomycin group, early in the course of the cyclosporin A-treated group, there was mild inflammatory morphology microscopically. The TGF-β concentration in BALF and expressions of FN and PDGF decreased more in the cyclosporin A-treated group than those in the bleomycin group. The pulmonary fibrosis in the cyclosporin A-treated group was almost disappeared on the 28th day. Conclusion Cyclosporin A may have an inhibitory effect on process of the pulmonary fibrosis through inhibiting expression of TGF-β,FN and PDGF.