小儿镇咳合剂中磷酸可待因的HPLC测定

HPLC DETERMINATION OF CODEINE PHOSPHATE IN ANTICOUGH MIXTURE FOR INFANT

在碱性溶液中经加盐和氯仿提取后采用ＨＰＬＣ法，用Ｓｈｉｍ－Ｐａｃｋ，ＣＬＣ－ＯＤＳＣ１８柱，以０．０１ｍｏｌ／Ｌ溴化四甲基铵溶液（ＨＯＡｃ调节ｐＨ＝３）－甲醇（４２∶５８）为流动相，能使小儿镇咳合剂中磷酸可待因得到很好的分离。平均回收率为１００．１％，ＲＳＤ为０．９５％。

Anticough mixture for infant is a complex preparation containing codeine phosphate and other active ingredients.A HPLC method with Shim Pack,CLC ODS C 18 column and 0.01 mol/L tetramethyl ammonium bromide (adjusting to pH3 with HOAc) methanol (42∶58) as the mobile phase was used for codeine phosphate determination after pretreatment with CHCl 3 extration in alkaline solution.The calibration curves showed a good linearity ( r =0.9999),The average recovery and the RSD were 100.1% and 0.95%,respectively.