菊苣农杆菌介导转化受体系统的研究(简报)

Research on a transformation acceptor system of Cichoriun intybus mediated by Agrobacterium tumefaciens

对影响菊苣转化受体系统几种重要因素进行了初步探讨。研究结果表明，采用菊苣叶片、茎段作为外植体，在MS＋6-BA1．5mg／L＋IBA0．2mg／L培养基上．再生频率达100％，农杆菌菌株LBA4404介导菊苣遗传转化体系中，经生根后20-25d的叶片．遗传转化效果最佳。共培养2d，同时附加20～25mg／L卡那霉素和500～600mg／L头孢霉素，转化频率由1．5％提高到7．1％左右。

Several important influential factors on a transformation acceptor system of Cichoriun intybus were studied and it was shown that the transformation frequency was 100%, when the leaves and caudexes as explants were cultivated on a co-culture medium of MS＋6-BA 1.5 mg/L＋IBA 0.2 mg/L. Furthermore, the genetic transformation of leaves for 20-25 d after rhizogenesis was most effective using the genetic transformation system of C. intybus mediated by Agrobocterium tumefaciens LBA4404. When the C. intybus leaves were co-cultured for 2 d with added ranamycim （20-25 mg/L） and cefotaxime （500-600 mg/L）, the transformation frequency increased from 1.5% to about 7.1%.