小鼠日本血吸虫病肝纤维化肝脏CTGF的表达及意义(英文)

Expression and significance of connective tissue growth factor in murine schistosomiasis liver fibrosis

目的研究结缔组织生长因子(connective tissue growth factor,CTGF)在日本血吸虫病肝纤维化小鼠肝脏中的表达状况及意义。方法用昆明小鼠感染尾蚴复制小鼠日本血吸虫病肝纤维化模型;RT-PCR和免疫组化分别检测小鼠肝脏CTGFmRNA和蛋白的表达,免疫组化方法观察各阶段肝脏α-SMA阳性细胞的表达。结果模型组10周时形成典型的肝纤维化,此时模型组小鼠肝脏CTGFmRNA和蛋白表达、α-SMA+细胞数达高峰,10周、14周、18周时与治疗组相比均有明显的统计学差异(P均>0.05);CTGFmRNA和蛋白表达水平与α-SMA+细胞数均呈直线相关性。结论CTGF基因和蛋白表达增高与小鼠日本血吸虫病肝纤维化形成有密切关系;CTGF在小鼠日本血吸虫病肝纤维化形成过程中的作用可能主要通过作用于活化的肝星状细胞来实现的。

[Objective] To study the expression and significance of connective tissue growth factor （CTGF） in murine schistosomiasis liver fibrosis. [Method] The mice infected with schistosoma japonicum were randomly divided into the model groups and the therapeutic groups. The hepatic expression of CTGF mRNA and protein was detected by revere transcriptase polymerase chain reaction and immunohistochemical method respectively. Histological changes were observed by HE staining. The α-SMA positive cells were observed by immunohistochemical method. [Result] The expression of CTGF mRNA and protein and α-SMA positive cells in the model groups peaked at 10th week and then declined; there were significant differences at 10th, 14th, 16th week between the model groups and the therapeutic groups at the same period respectively; the expression of α-SMA＋ positive cells had the same tendency as CTGF mRNA and protein. [Conclusion] The hepatic expression of CTGF is correlated to the murine schistosomiasis liver fibrosis; CTGF may take its biological effects by its action on HSCs.