I-Aβ_1基因与实验性小鼠膜性肾小球肾炎发病的相关性研究

THE STUDY ON RELATIONSHIP BETEEN I-AΒ_1 GENE AND PATHOLOGIC MECHANISM OF MEMBRANOUS GLOMERULONEPHRITIS IN EXPERIMENTAL MOUSE

目的:探讨I-Aβ1基因与实验性小鼠膜性肾小球肾炎发病之间的相关性。方法:复制并鉴定小鼠膜性肾小球肾炎动物模型,提取实验组和对照组总RNA,通过RT-PCR体外扩增I-Aβ1基因,扩增产物送北京三博远志测序中心测序,对测序结果进行分析。结果:实验组小鼠I-Aβ1基因突变率为2.578‰,对照组Aβ1基因突变率为0.286‰,实验组与对照组比较,实验组I-Aβ1基因突变率明显高于对照组(P﹤0.05),差异有统计学意义。结论:I-Aβ1基因在实验性膜性肾小球肾炎小鼠中杂合突变率增高,可能与小鼠膜性肾小球肾炎发生有关。

Objictive：To study the relationship between I-Aβ1 gene and the pathogenesis of membranous glomerulonephritis in experimental mouse. Methods：The animal model of MGN was reproduced and identified in mouse, then the total RNA of experimental group and control group was extracted. After I-Aβ1 gene was extract- ed,amplified and verified through PT-PCR,the PCR product was sent to the Beijing Sanboyuanzhi sequencing center to sequence the I-Aβ1 gene .The results of the sequencing was analysed. Results：The mutation rate of I- Aβ1 gene in experimental group was 2.578‰, while in the control group it is 0.286‰.The mutation rate of the experimental group is significatly exceeded that of the control group （P〈0.05）.Conciusions：The heterozygotic mutant rate of I-Aβ1 gene in experimental mouse is elevated ,which may relate to membranous glomerulonephritis.