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新Y染色体DYS726在山西汉族人群中遗传多态性 被引量：2

Polymorphism at DYS726 in a Chinese han population of Shanxi
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摘要 目的对新的DYS726基因座的基因多态性和法医学意义进行研究。方法山西汉族人群盅随机筛选110名男性,提取静脉血DNA,用基因数据库(GDB)中的引物进行聚合酶链反应(PCR)扩增,银染显色。对其筛选的等位基因测序,构建等位基因分型标准物;按照国际法医学会(ISFG)原则命名各等位基因。结果首次选择了Y染色体基因座DYS726,该基因座为4核苷酸简单重复STR。重复单位为CTTC。在110名山西汉族男性个体血样中共检出了4个等位基因。基因频率分别为11-0.0273;12-0.5455;13-0.1818;14-0.2455。基因多样性为0.6139,在法医学应用方面,其个人识别能力(DP)和非父排出率(PE)均为0.6139。结论新Y染色体基因座DYS726具有较高的遗传多态性,在法医学及人类遗传学方面具有较高应用价值。 Objective To investigate the gene polymorphism and the utility of a new locus DYS726. Methods Each allele of this Y-STR loci was sequenced and the allele ladders were constructed. Alleles of this Y-STR locus were nominated according to reconmaendations of the International Society of Forensic Genetics. Results We first choose the Y-STR locus DYS726. The number of the simple repeat nucleotide is four and the sequence is CTTC. Four alleles had been found in 110 unrelated male venous blood in Chinese Han population of Shanxi. The frequencies of throe locus are 11 - 0. 027 3 ; 12 - 0. 545 5 ; 13 - 0. 181 8 ; 14 - 0. 245 5. If used in forensic science, both of the power of discrimination （PD） and the power of exclusion （PE） were 0. 6139. Conclusion DYS726 has a high level of genetic polymorphism and can be used in forensic science and human genetics research.
作者 吴晓兵 郝旭宇 杨思思 郭大玮 张更谦
机构地区 山西医科大学
出处 《山西医药杂志（上半月）》 CAS 2008年第2期99-100,共2页 Shanxi Medical Journal
基金 山西省自然科学基金资助项目(20051046)
关键词 Y染色体 山西 汉族 短串联重复序列 STR DYS726 Y chromosome Shanxi Han nationality Short tandem repeat STR DYS726
分类号 R394 [医药卫生—医学遗传学] R698.2 [医药卫生—泌尿科学]
  • 相关文献

参考文献9

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共引文献34

同被引文献12

  • 1赵瑞君,赵仰星,李晋华,张伟华,梁景青,唐泽忠,尹镭,李国锦,殷国荣.TKM──血液DNA快速提取法[J].山西医学院学报,1996,27(1):4-6. 被引量：29
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  • 6Gill P , Brenner C , Brinkmann B, et al. DNA Commission of the International Society of Forensic Genetics: recommendations on forensic analysis using Y - chromosome STRs[J]. Forensic Sci Int,2001 ,(24) :5 - 10.
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  • 10Tie J,Wang X,Suzuki Y,Tsukamoto S,Oshida S. Polymorphlsms of sixY - chromosome STRs in a Chinese population[J]. Legal Med,2004, (6) :41 -46.

引证文献2

二级引证文献1

山西医药杂志（上半月）
2008年 第2期

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