载脂蛋白E基因多态性与颈动脉粥样硬化及相关炎症因子产生的关系

The relationship among apolipoprotein E genetic polymorphism,carotid atherosclerosis and the related inflammatory factors

目的:探讨载脂蛋白E(ApoE)的基因多态性与颈动脉粥样硬化(CAS)及相关炎症因子产生的关系。方法:入选的83例对象均行彩色多普勒检测颈动脉内中膜厚度(IMT)及观察颈动脉有无斑块,根据结果分成CAS组及对照组,采用聚合酶链式反应-限制性片段多态性方法检测ApoE基因多态性,并测定总胆固醇(TC)、甘油三脂(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)及相关炎症因子。结果:CAS组ε3/4基因型频率明显高于对照组(P<0.05),两组间ε4等位基因频率差异有显著性(P<0.05)。不同的ApoE等位基因组间TC、LDL-C、TG、颈动脉IMT、超敏C反应蛋白(Hs-CRP)和纤维蛋白原(FIB)存在显著性差异(P<0.05),并且上述指标的值以携带ε4等位基因组为最高。结论:ApoE基因多态性是影响血清TC及LDL-C水平的重要遗传因素之一,同时与体内炎症状态相关,并且在一定程度上影响CAS病变的进程。

Objective：To explore the relationship among apolipoprotein E（ApoE） genetic polymorphism, the carotid atherosclerosis （CAS） and the produce of related inflammatory factors. Methods：Total 83 patients were examined by B-mode ultrasound to measure intima-media thickness （IMT） of the common carotid artery and plaque formation. According to the results of B-mode Ultrasound examination,the patients were divided into the carotid atherosclerosis group and control group. The polymerase chain reaction restriction fragment length polymorphism technology （PCR-RFLP） was used to detect ApoE genotype; Serum triglyceride （TG）,total cholesterol （TC）,low density lipoprotein cholesterol （LDL-C）,high density lipoprotein cholesterol （HDL-C） and inflammatory factors were measured. Results：The frequency of ε3/4 in CAS group was higher than that in control group（P 〈 0.05）. The values of TC ,LDL-C,TG, carotid IMT,Hs-CRP and fibrinogen among different groups of ApoE allele were significantly different （P 〈 0.05）,and the value of these parameters in the group carrying ε4 allele ranked the highest among all genetic polymorphism. Conclusion： ApoE genetic polymorphism was one of the important genetic factors affecting serum total cholesterol and LDL-C level. Meanwhile correlating with the state of inflammation in vivo, ApoE genetic polymorphism also affected CAS process in a certain extent.