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热休克凋亡乳腺癌细胞负载的DC体内激发Th1型免疫反应 被引量：5

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摘要 目的:探讨乳腺癌患者免疫热休克凋亡乳腺癌细胞负载的树突状细胞(DC)后体内免疫状态的变化,并对其安全性进行初步评估。方法:分离患者外周血单核细胞,用重组人粒细胞-巨噬细胞集落刺激因子(rhGM-CSF)和白介素-4(rhIL-4)诱导DC;将患者自体肿瘤细胞热休克后诱导其凋亡并负载自体DC。对10例确诊为乳腺癌的患者进行1个疗程(4次)的DC免疫治疗。结果:治疗中未出现明显不良反应,生活质量均有不同程度的改善,血清中IL-2、IL-12、IFN-γ和TNF-α水平治疗后呈明显上升趋势。结论:热休克凋亡乳腺癌细胞负载的DC体内可激发Th1型免疫应答,增强机体抗瘤能力,同时无明显毒副反应。
作者 朱玉兰 钱科卿 高海燕 许凌云 钱琦 陆晓 宁永玲
机构地区 南京医科大学附属常州第二人民医院乳腺外科 南京医科大学附属常州第二人民医院肿瘤科 南京得康生物技术有限公司
出处 《南京医科大学学报（自然科学版）》 CAS CSCD 北大核心 2008年第1期113-116,共4页 Journal of Nanjing Medical University(Natural Sciences)
基金 常州市社会发展基金资助(CS2007215)
关键词 乳腺癌 树突状细胞 肿瘤免疫治疗
分类号 R737.9 [医药卫生—肿瘤]
  • 相关文献

参考文献7

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共引文献12

同被引文献45

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引证文献5

二级引证文献15

南京医科大学学报（自然科学版）
2008年 第1期

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