Characterization of a Mutant of Alteromonas aurantia A18 and Its Application in Mariculture

Mutant J61321 with enhanced siderophore production of Alteromonas aurantia A18 was obtained after a series of chemical-physical mutageneses. It was found that the antibacterial activity against Vibrio anguillarum W-1 and siderophore produc- tion of the mutant were higher than those by the original strain A18 which had been used in mariculture. The results of the specific assay(Csáky and Arnow methods) of siderophore showed that the sidrophore with hydroxamate group was produced by mutant J61321 and the original strain A18, respectively, while the siderophore with catechol group was yielded by strain W-1 (Aibrio an- guillarum). Meanwhile, the siderophore yield, antibacterial activity and anti-chelator activity of strain J61321 were higher than those of its parent strain A18.

Mutant J61321 with enhanced siderophore production of Alteromonas aurantia AI8 was obtained after a series of chemical-physical mutageneses. It was found that the antibacterial activity against Vibrio anguillarum W-1 and siderophore production of the mutant were higher than those by the original strain A 18 which had been used in mariculture. The results of the specific assay（Csaky and Arnow methods） of siderophore showed that the sidrophore with hydroxamate group was produced by mutant J61321 and the original strain A 18, respectively, while the siderophore with catechol group was yielded by strain W-1 （Aibrio anguillarum）. Meanwhile, the siderophore yield, antibacterial activity and anti-chelator activity of strain J61321 were higher than those of its parent strain A 18.