戊四氮点燃模型大鼠海马苔藓纤维出芽的动态变化

Changes of mossy fiber sprouting in hippocampus of pentylenetetrazole kindling rats

目的:研究戊四氮(pentylenetetrazole,PTZ)慢性致癎模型点燃前后大鼠海马苔藓纤维出芽的动态变化,以探讨癫痫可能的发病机制。方法:将60只大鼠随机分为对照组和PTZ组(30mg/kg腹腔注射,每天1次)。在点燃前后用Timm染色法观察海马苔藓纤维出芽的动态变化。结果:PTZ组大鼠在行为学和脑电图尚未出现癎性改变之前就有苔藓纤维出芽,且随着点燃效应的逐步建立,苔藓纤维出芽逐渐增强。结论:苔藓纤维出芽可能对癫痫的形成和发展起重要作用。

Objective To determine the changes of mossy fiber sprouting in hippocampus of pre-kindling and post-kindling rats of chronic epilepsy induced by pentylenetetrazole（PTZ）.Methods Sixty rats were randomly divided into a control group and a PTZ group（PTZ 30 mg/kg,intraperitoneal injection,once daily）.The changes of mossy fiber sprouting in hippocampus of pre-kindling and post-kindling rats were examined by Timm staining.Results Before the occurrence of convulsion confirmed by behavior and EEG,the mossy fiber sprouting was found in the PTZ group.The grade of the mossy fiber sprouting increased with the gradual establishment of kindling effect.Conclusion Mossy fiber sprouting may play an important role in the onset and development of epilepsy.