摘要
用高效液相色谱法测定红花籽中牛蒡子苷的含量。其色谱条件为：采用Waters C18柱（150mm×3．9mm，5μm），流动相为乙腈-0．05％磷酸溶液（25：75），流速0．6mL／min，检测波长210nm，柱温室温，用外标法定量，测定红花籽中牛蒡子苷的含量。牛蒡子苷的线性范围0．032—0．16μg，R=0．9994，回收率101．50％，RSD2．81％。该方法简便，快速，线性关系良好，可作为牛蒡子苷的含量测定。
The purpose is to determine the content of aretiin in Carthamus tintorius L. seed. The analysis was carried out on Waters C18 column（ 150 mm × 3.9 mm ,5μm）. The mobile phase was acetonitrile-0.05% H3PO4 （25：75 ）. Flow-rate was 0.6 mL/min. Wave-length was 210 nm. Temperature was room temperature. The liner range of aretiin was 0.032- 0.16 μg and gave a correlation（r） of 0. 9994. The recovery was 101.50%. RSD was 2.81%. The method was simple, fast and had a good liner relationship. It is to determine the content of aretiin in Carthamus tinctorius L. seed.
出处
《天然产物研究与开发》
CAS
CSCD
2007年第B05期87-89,共3页
Natural Product Research and Development
