紫花苜蓿中叶黄素超声提取优选工艺条件的研究

Study on the Ultrasonic Extraction Process of Lutein from Alfalfa

采用L9（3^4）正交试验，以叶黄素含量为评价指标，用紫外可见分光光度法进行测定，考察了超声温度（A）、时间（B）、超声功率（C）、超声频率（D）对超声波提取苜蓿中叶黄素的影响。结果表明：优化工艺为提取温度55℃，提取时间45min，超声波功率50W，超声频率45kHz。该工艺合理，可大大缩短工时，提高收率。

According to the orthogonal design L9 （3^4） , the four relevant factors：ultrasonic temperature （A）, ultrasonic time （B） ,ultrasonic power （C） ,ultrasonic wave frequency （D） have been investigated. Taking the determination of lutein as a criterion,the content of lutein has been defined by UV-Vis method. The results showed that the optimum ultrasound extracting procedure was ： ultrasonic temperature 55 ℃, ultrasonic time 45 min, ultrasonic power 50, ultrasonic wave frequency 45 kHz. It is a reasonable technique,the process was simple and reliable.