白木香的遗传差异及ISSR分子鉴定

Genetic Variation and Molecular Identification of Aquilaria Sinensis by ISSR

目的采用简单重复序列区间（ISSR）扩增技术，对不同地区白木香的遗传变异、亲缘关系及分子鉴定进行研究。方法筛选随机引物进行ISSR扩增，通过UPGMA聚类，研究白木香各居群间的遗传关系，构建居群亲缘关系的分子系统树；利用特异性条带对白木香进行指纹分析鉴别。结果共筛选出7条有效引物，经ISSR扩增得到80条带，平均每条引物扩增出11．4条带，其中多态性条带47条，多态性程度达58．8％，居群间遗传距离为0．0733～0．4213。结论ISSR分子标记可作为白木香居群遗传多态性、居群亲缘关系和分子鉴别的有效手段；引物IS-8可以进行白木香种内和种间的鉴别。

Aim To investigate the genetic diversity, relationship and molecular authentication of different populations ofAquilaria Sinensis using ISSR markers. Methods 7 random primers were screened for inter simple sequence repeat （ISSR） fragments. A DNA molecular dendrogram was established based on cluster analysis by UPG-MA （unweighted pair-group method with arithmetic average） , and all the populations were authenticated. Results A total of 80 bands was amplified with 7 ISSR primers, in which 47 were polymorphic, corresponding to 58.8% genetic polymorphism. The genetic distances were 0.073 3 to 0.421 3 between populations. Conclusion ISSR markers were an informative and useful tool for the genetic diversity, evaluation and authentication of A. sinensis. Primer IS8 could be used to authenticate the populations of A. sinensis and their adulterants.