摘要
为建立香苓胶囊的含量测定方法,采用苯酚硫酸法,以葡萄糖作为标准品,分光光度法于490砌波长处测定吸光度。实验显示多糖的线性范围为2—10μg,/mL,r=0.9997,加样回收率为99.81%(n=5),RSD=1.95%,样品中多糖的质量分数为89.22%,表明该方法可用于本品的质量控制。
To develop a method for the determination of polysaccharide in Xiangling capsule, total polysaccharide were determined by phenol-sulfurlc acid method with glucose as the standard, and spectrophotometer assay absorbency at 490 nm. Results showed that the linear range of polysaccharide was 2-10 μg/mL, r = 0. 9997 and the recovery rate was 99. 81% (n=5),RSD = 1.95%. Through sample assay,the content of polysaceharide was 89.22%. It suggested that this method can be used as the quality control of this preparation.
出处
《天然产物研究与开发》
CAS
CSCD
2007年第B08期260-261,共2页
Natural Product Research and Development
基金
黑龙江省教育厅研究生创新资助项目
黑龙江“研究生培养创新计划”项目(YJSCX2005-127HLJ)
关键词
香苓胶囊
多糖
苯酚硫酸法
Xiangling capsule
polysaccharides
methodology
phenol-sulfuric acid method