香苓胶囊中多糖的含量测定

Determination of Polysaccharide in Xiangling Capsule

为建立香苓胶囊的含量测定方法，采用苯酚硫酸法，以葡萄糖作为标准品，分光光度法于490砌波长处测定吸光度。实验显示多糖的线性范围为2—10μg,／mL，r=0．9997，加样回收率为99．81％（n=5），RSD=1．95％，样品中多糖的质量分数为89．22％，表明该方法可用于本品的质量控制。

To develop a method for the determination of polysaccharide in Xiangling capsule, total polysaccharide were determined by phenol-sulfurlc acid method with glucose as the standard, and spectrophotometer assay absorbency at 490 nm. Results showed that the linear range of polysaccharide was 2-10 μg/mL, r = 0. 9997 and the recovery rate was 99. 81% （n=5）,RSD = 1.95%. Through sample assay,the content of polysaceharide was 89.22%. It suggested that this method can be used as the quality control of this preparation.