摘要
研究了用高效液相色谱法快速测定桑叶中的糖类物质的方法。色谱泵为LC-10ATvp高压恒流泵。检测器为RID-10A示差折光检测器，色谱柱为Agilent ZORBAX NH24．6×250mm，柱温40qc，检测池温度为40℃，流动相为乙腈：水=75-25，流速1．0mL/min，进样体积10曲。结果表明，此方法测定各种单糖的稳定性好，用相对标准偏差RSD表示，鼠李糖3．33％，木糖0．28％，果糖0．62％，葡萄糖0．34％，蔗糖3．41％，麦芽糖2．26％，乳糖3．09％，棉子糖0．19％。该方法快速简捷，精密度高，重现性好。
Determination of carbohydrates in mulberry leaves was studied by HPLC. The system of chromatography included the high-press constant-current of LC-10ATvp,the refractive index detector, RID-10A ,and the chromatographic column of Agilent ZORBAX NH2 4.6×250mm. The terperature in column and tetector was 40℃. The flowing phase was CHa CN：H2O=75：25,with a flow rate of 1.0 mL/min. The input volume was 10μL. The result showed that the stability of rhamnose, xylose, fructose, glucose, sucrose, maltose, lactose and raffinose were 3.33%, 0.28%, 0. 62%,0.34% ,3.41% ,2.26% ,3.09% and 0.19% respectively. The method was accurate,rapid,simple and reproducible.
出处
《天然产物研究与开发》
CAS
CSCD
2007年第B08期268-270,共3页
Natural Product Research and Development
关键词
桑叶
高效液相色谱
糖类
mulberry leaves
HPLC
carbohydrates
