盾叶薯蓣中薯蓣皂甙元提取新工艺的研究

A New Technology for Extracting Diosgenin from Dioscorea zingibersis C. H. Wright

为研究新工艺提取盾叶薯蓣薯蓣中薯蓣皂甙元的最佳实验指标，以薯蓣皂甙元得率为评价参数，采用6因素5水平的正交实验，用分光光度法对25种提取方法所得到的薯蓣皂甙元进行比较分析。结果表明，硫酸浓度对薯蓣皂甙元提取的影响最大，在实验室条件下，可采用20g样品加甲醇回流提取4h，回流速度为10min／次，用2．5mol／L的硫酸水解6h，120号溶剂汽油回流提取2h，回流速度为15min／次，能清洁快速提取盾叶薯蓣中的薯蓣皂甙元。

To research a new technology for extracting diosgenin from Dioscorea zingibersis C. H. Wright, several important factors were analyzed in the orthogonal study of six factors and five levels,the content of diosgenin was valued as an evaluation index using spectrophntometry. The results indicated that the concentration of sulfuric acid has an obvious effect on the content of diosgenin extracted. And experiments showed that the optimal technology for diosgenin extraction is under the conditions shown as followings ：20 g sample is refluxed for 4 h with methanol, and 10 min for each reflux operation;then 100 mL 2.5 mol/L H2 SO4 is added for 6 hours of hydrolysis;at last is refluxed for 2 h with gasoline, and 15min for each reflux operation,which can be used as a rapid and clean extraction technology.