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基于硒化镉量子点磁微球的微悬臂梁式免疫传感器片上磁分离 被引量:1

On-chip Magnetic Separation of Microcantilever Immunosensor Based on the CdSe QDs-tagged Magnetic Microbead
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摘要 应用了以硒化镉量子点为荧光探针,具有磁性和抗体双重靶向功能的聚苯乙烯磁微球。设计了基于此种磁微球的新型微悬臂梁式免疫传感器,满足在液相环境中,借助嵌入到聚苯乙烯磁微球的荧光探针及微球表面的特异性抗体探针,达到生物分子的定性检测,借助具有纳米机械响应的微悬臂梁及微平面电感线圈,达到生物分子的定量检测及传感器的复用性,解决传统微悬臂梁式免疫传感器的不足。着重对三种粒径尺寸的硒化镉量子点进行了表征,同时针对片上磁分离的机理,梁上微电感线圈的结构,微磁场对磁微球的吸引进行了研究,设计并优化出满足新型微悬臂梁式免疫传感器所需的蛇形微平面电感线圈。通过生物磁分离实验,验证了设计及优化的结果,实现了用于生物分子分离的片上磁分离技术。 We designed a novel microcantilever immuosensor based on magnetic microbead, applying different-sized CdSe QDs as fluorescent probes and polystyrene magnetic microbead. The novel microcantilever immuosensor used fluorescent probes embedded polystyrene microbeads and specific antibodies on the surface of the polystyrene microbead. In addition, we studied the mechanism of the on-chip magnetic separation, the structure of micro-electromagnet and the microbead magnetization by the micro-magnetic field, the snake-shaped planar micro-electromagnet for the novel microcantilever immuosensor.
出处 《生物工程学报》 CAS CSCD 北大核心 2008年第2期315-322,共8页 Chinese Journal of Biotechnology
基金 国家自然科学基金(No.69876027)和天津市自然科学基金重点项目(No.075CZDJC10400).
关键词 免疫传感器 硒化镉 量子点标记 磁微球 片上磁分离 生物分子 immunosensor, CdSe Quantum dots (QDs)-tagged microbead, on-chip magnetic separation
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参考文献12

  • 1Boisen A, Thaysen J, Jensenius H. Environmental sensors based on micromachined cantilevers with integrated read-out. Ultramicroscopy, 2000, 82 (1-4): 11-16.
  • 2Larry R Senesac, Pampa Dutta, Panos G Datskos. Sepaniak Analyte species and concentration identification using differentially functionalized microcantilever arrays and artificial neural networks. Analytica Chimica Acta, 2006, 558 (1-2): 94-101.
  • 3Tartaj P, Morales ME Sema T Gonzalez-Carreno. Advances in magnetic nanoparticles for biotechnology applications. Journal of Magnetism and Magnetic Materials, 2005, 290 (1): 28-34.
  • 4J Justin Gooding. Biosensor technology for detecting biological warfare agents: Recent progress and future trends. Analytica Chimica Acta, 2006, 559 (2): 137-151.
  • 5Datskos PG, Sepaniak M J, Tipple CA. Photomechanical chemical mierosensors. Sensors and Actuators B: Chemical, 2001, 76(1-3): 393-402.
  • 6Rick Archibald, Panos Datskos, Gerald Devault. Independent component analysis of nanomechanical responses of cantilever arrays. Analytica Chimica Acta,2007,584(1): 101-105.
  • 7Headrick J J, Sepaniak M J, Lavrik NV. Enhancing chemi-mechanical transduction in microcantilever chemical sensing by surface modification. Ultramicroscopy, 2003, 97 (1-4): 417-424.
  • 8Yang M, Yau HCM, Chan HL. Adsorption kinetics and ligand-binding properties of thiol-modified double stranded DNA on gold surface. Langmuir, 1998, (14): 6121-6129.
  • 9Lee Jeongwoo, Ha Jin Uk, Choe Soonja. Synthesis of highly monodisperse polystyrene microspheres via dispersion polymerization using an amphoteric initiator. Journal of Colloid and Interface Science, 2006, 298 (2): 663-671.
  • 10Li Zhaohui, Wang Kemin, Tan Weihong, Li Jun, Fu Zhiying. Immunofluorescent labeling of cancer cells with quantum dots synthesized in aqueous solution. Analytical Biochemistry, 2006, 354(2): 169-174.

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