高效液相色谱法测定甲亢灵颗粒中丹参素的含量

Determination of Danshensu in Jiakangling Granules by HPLC

目的反相高效液相色谱法测定甲亢灵颗粒中丹参素的含量。方法反相高效液相色谱法。采用Hypersil BDS C18色谱柱；以甲醇-1％冰醋酸溶液（10：90）为流动相；检测波长280nm；流速1．0ml／min；柱温25℃。结果丹参素钠在3．612-18．06μg／ml浓度范围内线性关系良好，回归方程：y=22676x-73264，r=0．9999，平均回收率为98．9％，RSD为1．7％（n=5）。通过含量测定，确定甲亢灵颗粒每袋含丹参素（C9H10O5）不得少于9．9mg。结论该法操作简便，结果准确、可靠，为甲亢灵颗粒的质量控制提供依据。

Objective To determine Danshensu in Jiakangling Granules by HPLC. Methods HPLC analysis was applied to determine Danshensu in Jiakangling Granules. The HPLC chromatographic conditions included Hypersil BDS column; the mobile phase was methanol-1% glacial acetic （10：90） and the detective wavelength was at 280 nm,column temperature was 25℃. Results There were good linear relationship for Dan-shensu within the range of 3. 612-18.06 μg/ml,r=0.9999. The average recovery was 98.9% ,RSD=1.7% （n=5）. Conclusion The methods were of good accurate and a simple procedure. It may be practical to control the quality of Jiakangling Granules.