气腹预处理对腹腔镜手术大鼠肝脏保护作用的实验研究

Animals Experimental Study on Pneumoperitoneum Preconditioning Protection on Liver in Laparoscopy

目的:探讨气腹预处理(充气10min/放气10min)对腹腔镜手术大鼠的肝脏保护作用。方法:将24只雄性SD大鼠随机分成三组:直接气腹组、气腹预处理组和正常对照组。三组动物的麻醉、气腹孔准备等相同。正常对照组不气腹;直接气腹组以CO2充气60min/放气30min;气腹预处理组先行CO2充气10min/放气10min预处理后再以CO2充气60min/放气30min。实验完成后留取血样本和肝脏标本分别进行血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、肝组织脂质过氧化物丙二醛(MDA)含量测定和肝脏的组织形态学观察,并统计分析各组间差异。结果:直接气腹组、气腹预处理组ALT、AST、MDA水平均明显高于正常对照组,但气腹预处理组较直接气腹组明显降低,三组间差异有显著性。三组间组织形态学变化亦有差异。结论:气腹过程对肝脏具有损伤作用;气腹预处理对气腹所产生的肝脏损伤具有保护作用。

Objective:To study the protective effect of pneumoperitoneum preconditioning on liver in laparoscopy.Method:The subjects were 24 Sprague-Dawlay male rats which were randomizedly divided into three groups:Pneumoperitoneum(P)group,preconditioning(PRE)group,contral(C)group.pneumoperito-neum was created in all except C group.PRE consisted of 10 min of P,followed by 10 min of deflation(D).Group P was subjected to 60 min of P,followed by 30 min of D.Group PRE was subjected to PRE,followed by 60 min of p and 30 min of D.At the end of the experiment,the rats were killed.Blood samples and liver samples were obtained and coded.Plasma alanine aminotransferase(ALT),aspartate aminotransferase(AST)and liver tissue malondialdehyde(MDA)as well as histopathological evidence were assessed and analyzed.Results:Plasma ALT and AST as well as liver MDA levels were markly increased in group P and group PRE,as compared to group C.It was higher in group P than group PRE.The difference between group P and group PRE was considered significant.Histopathological evidences in three groups were different.Conclusion:P can bring liver injury in rats.PRE can improve liver function in rats in laparoscopy.