新生大鼠缺氧缺血性脑损伤时Bcl-2蛋白表达的研究

Study on the expression of bcl-2 protein after hypoxic ischemic brain damage in neonatal rats

目的:通过研究新生大鼠脑缺血时bcl-2蛋白在海马区的表达特点,探讨bcl-2蛋白在缺血缺氧性脑损伤(HIBD)的作用方法:56只7日龄新生SD大鼠随机分为1个对照组和7个实验组。给动物吸入含有92%氮气和8%氧气混合气体建立新生大鼠HIBD动物模型,分别在脑损伤后不同时间点(0.5、1、3、6、12、24、48、72h等)断头处死动物,取海马组织,用免疫组织化学方法检测海马脑神经细胞凋亡及bcl-2蛋白表达情况。结果:TUNEL染色的阳性细胞数与bc1-2蛋白阳性的细胞数在HIBD后的不同时间点出现分别有显著差异,海马区bcl-2蛋白阳性细胞在HIBD后立即出现,6h达到高峰,之后渐下降。结论:bcl-2蛋白强表达于海马缺血区和缺血周边区的神经元,与神经元的存活或死亡有一定联系。

Objective ：To study the effect of bel-2 gene in cell apoptosis by observing the expression of bcl-2 gene in hippocampus of hypoxic ischemic brain damage（HIBD）. Methods：Fifty-six neonatal rats were used in a control group and 7 trial groups.The models of HIBD were established in neonatal rats by inhaling the mixed gases of 92% N2 and 8%O2,and the animals were sacrificed by dislocating their heads at different time morphological analysis.Results：The positive cell of be1-2 protein increased after HIBD and reached the peak at 6h and then decreased gradually following HIBD.Conclusion：The expression of bcl-2 gene in hippocampus is stronger than that of the other areas of brain, and there is relationship between cell apoptosis and bcl-2 gene.