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63例非小细胞肺癌组织Polo样激酶1的表达及其意义

Expression of Polo-like Kinase 1 in 63 Cases with Non-small Cell Lung Cancer and its Significance
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摘要 [目的]研究非小细胞肺癌中Polo样激酶1(PLK1)的表达及其临床意义。[方法]采用免疫组化Envision两步法技术,检测63例非小细胞肺癌手术切除标本中的PLK1和p53表达水平。另取10例距肺癌5cm的癌旁正常肺组织作对照。[结果]PLK1在非小细胞肺癌组织中的总表达率为79.4%(50/63),10例癌旁正常肺组织无表达(χ2=21.65,P<0.01)。PLK1在非小细胞肺癌中的表达与肿瘤的病理类型、组织分化程度无明显相关(χ2=0.39、1.60,P>0.05);PLK1表达的阳性率与有无淋巴结转移相关(χ2=5.36,P<0.05),与淋巴结转移的远近无关(χ2=0.59,P>0.05);PLK1的表达在Ⅰ、Ⅱ、Ⅲa期肿瘤中差异有显著性(χ2=6.84,P<0.05),随着分期的进展,PLK1表达的阳性率也逐渐增高;PLK1的表达与p53蛋白积聚呈显著相关(χ2=5.99,P<0.05)。[结论]PLK1在非小细胞肺癌中高表达,与抑癌基因p53的积聚明显相关,在非小细胞肺癌发生发展中起重要作用,并与预后相关。
作者 叶敏华 周涛 卢洪胜 蔡菊芳 甘梅富 马德华
机构地区 浙江省台州医院
出处 《中国肿瘤》 CAS 2008年第2期146-148,共3页 China Cancer
关键词 非小细胞肺癌 POLO样激酶1 P53
分类号 R734.2 [医药卫生—肿瘤]
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参考文献13

  • 1Cogswell JP, Brown CE, Bisi JE, et al. Dominant-negative polo-like kinase 1 induces mitotic: catastrophe independent of cdc25C function[J]. Cell Growth Differ, 2000, 11(12): 615-623.
  • 2Toyoshima-Morimoto F, Taniguchi E, Shinya N, et al. Polo-like kinase 1 phosphorylates eyclin B1 and targets it to the nucleus during prophase[J]. Nature, 2001, 410(6825): 215-220.
  • 3Casenghi M, Meraldi P, Weinhart U, et al. Polo-like kinase 1 regulates Nlp, a centrosome protein involved in mierotubule nucleation[J]. Dev Cell, 2003, 5(1): 113-125.
  • 4Sumara I, Vorlaufer E, Stukenberg PT. The dissociation of cohesin from chromosomes in prophase is regulaled by Polo-like kinase[J]. Mol Cell, 2002, 9(3):515-525.
  • 5Lee KS, Yuan YL, Kuriyama R, et al. Plk is an M phasespecific protein kinase and interacts with a kinesinlike protein, CHO1/MKLP-1[J]. Mol Cell Biol, 1995, 15(12): 7143-7151.
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  • 8应可净,邵方淳,余碧芸,吴金民,丁佳逸.肺癌活检组织中Polo样激酶-1mRNA水平检测的临床意义[J].中华检验医学杂志,2006,29(9):813-816. 被引量：2
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二级参考文献15

  • 1Glover DM,Hagan IM,Tavares AA.Polo-like kinases:a team that plays throughout mitosis. Genes Dev, 1998, 12:3777-3787.
  • 2Nigg EA. Polo-like kinases: positive regulators of cell division from start to finish. Curr Opin Cell Biol, 1998, 10: 776-783.
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  • 4Livak K J, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C (T))Method. Methods,2001,25:402-408.
  • 5Toyoshima-Morimoto F, Taniguchi E, Shinya N, et al. Polo-like kinase 1 phosphorylates cyclin B1 and targets it to the nucleus during prophase. Nature, 2001,410: 215-220.
  • 6Casenghi M, Meraldi P, Weinhart U, et al. Polo-like kinase 1 regulates Nip, a centrosome protein involved in microtubule nucleation. Dev Cell, 2003, 5: 113-125.
  • 7Lane HA, Nigg EA. Antibody microinjection reveals an essential role for human polo-like kinase 1 ( Plk1 ) in the functional maturation of mitotic centrosomes. J Cell Biol, 1996, 135: 1701-1713.
  • 8Spankuch-Schmitt B, Bereiter-Hahn J, Kaufmann M, et al. Effect of RNA silencing of polo-like kinase-1 (PLK1) on apoptosis and spindle formation in human cancer cells. J Natl Cancer Inst, 2002,94: 1863-1877.
  • 9Wolf G, Elez R, Doermer A, et al. Prognostic significance of polo-like kinase (PLK) expression in non-small cell lung cancer.Oncogene, 1997, 14: 543-549.
  • 10Knecht R, Elez R, Oechler M, et al. Prognostic significance of polo-like kinase (PLK) expression in squamous cell carcinomas of the head and neck. Cancer Res, 1999, 59: 2794-2797.

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中国肿瘤
2008年 第2期

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