摘要
为进一步明确桃蚜与其内共生菌Buchnera之间系统进化关系,参照前人研究蚜虫系统进化所用的COⅠ/COⅡ、12S/16S rRNA、EF-1 alpha的3对引物,对桃蚜的COⅠ/COⅡ、12S/16S rRNA、EF-1 alpha基因进行了扩增,并构建了桃蚜COⅠ/COⅡ、12S/16S rRNA、EF-1 alpha基因序列系统进化树。通过分析发现,桃蚜的COⅠ/COⅡ、12S/16SrRNA基因序列均与已知的麦双尾蚜(Diuraphis noxia(Kurdj))的相应基因序列最近,而桃蚜与麦双尾蚜的EF-1 alpha基因序列则处于相邻的分支。综合我们以前的研究分析桃蚜及其内共生菌Buchnera的系统进化关系,发现桃蚜与其内共生菌是协同进化的。
To further explore the phylogenetic relationships between the aphid Myzus persicae and its endosymbiotic bacterium Buchnera, COⅠ/COⅡ , 12S/16S rRNA and EF-1 alpha genes in four populations of M. persicae were amplified. Through phylogenetic trees constructed by the COⅠ/COⅡ , 12S/16S rRNA and EF-1 alpha genes in M. persicae, we found that the COⅠ/COⅡ and 12S/16S rRNA genes sequences in M. persicae were more closely related to those in Diuraphis noxia, while the EF-1 alpha genes of M. persicae and D. noxia located in two neighboring branches. We conclude that the phylogeny of Buchnera and its host M. persicae are co-evolutional based on our previous and present studies.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2008年第1期51-56,共6页
Journal of Nanjing Agricultural University
基金
国家863计划项目(2002AA244041)