冷却肉中乳酸含量的反相高效液相色谱分析

Determination of lactic acid content in chilled meat by RP-HPLC

为测定冷却肉中乳酸含量，建立了冷却肉中乳酸提取及反相高效液相色谱的测定方法。冷却猪肉用1mol·L^-1高氯酸水溶液匀浆去除蛋白质及脂肪后，5000g离心，并过0．25μm纤维素膜，然后以高效液相色谱反相柱分离，通过紫外检测器检测。检测结果显示，该测定方法回收率为95．5％-102．3％，相对标准偏差为3．2％-5．7％，日内测定变异系数（CV）为1．09％，日间测定CV为1．28％。该方法应用于冷却猪肉乳酸含量测定获得较好的效果。

A reversed-phase high performance liquid chromatography （RP-HPLC） method was established to determine lactic acid content in chilled meat. The samples pretreated with the solution of 1 mol · L^-1 perchloric acid （PCA） was separated through RP- HPLC. The determined result indicated that the recovery rate was 95.5%- 102. 3%, and relative standard deviation was 3.2%- 5.7% , The coefficient of variance within-day and between-day was 1.09% and 1.28% respectively. The method was successfully used in the analysis of chilled pork.