影响黄瓜未授粉子房培养胚发生因素的研究

Factors affecting embryo formation in unpollinated ovary culture of cucumber

以不同基因型黄瓜为试材，研究了子房发育时期、热激处理时间、TDZ和AgNO3对黄瓜未授粉子房培养过程中胚发生的影响。结果表明：处于开花前2～3d的子房胚发生率相对较高，达83．8％；在子房培养开始阶段进行35℃处理的胚发生率显著高于对照（0d），其中处理3d的效果最好；添加0．01～0．04mg·L^-1 TDZ的诱导培养基培养的胚发生率为20．0％～72．7％，其中添加0．04mg·L^-1 TDZ的胚发生效果较好；在诱导培养基中添加AgNO3可以提高胚发生率，同时能缩短胚出现的时间并提高胚产量。

Different genotypes of cucumber were used to study the effects of development stage of ovaries, time of thermal treatment, and concentration of TDZ and AgNO3 on the unpollinated ovary culture of cucumber. The results showed that the highest frequency 83. 8% of embryo formation was obtained as using ovaries 2 - 3 d before anthesis. Embryo formation after thermal shock treatment at 35℃ was significantly higher than control （0 d）, and highest embryo formation was found in treating three days. Embryo formation frequencies were from 20.0% to 72.7% when TDZ was added from 0. 01 -0. 04 mg · L^-1 to inducting medium, and the best result was obtained at 0.04 mg · L^-1 TDZ. Adding AgNO3 to inducting medium could increase embryo formation frequency, and shorten the time for embryo sprouting and enhance the embryo yields.