鸡IL-18对IBDV多聚蛋白DNA疫苗的免疫增强作用研究

Immunoenhancement on DNA vaccine of polyprotein gene in infectious bursal disease virus by co-delivery with plasmid encoding chicken interleukin-18.

为进一步评价鸡IL-18的免疫增强作用,用鸡白细胞介素18(IL-18)的真核表达质粒(pCI-IL-18)与传染性法氏囊病病毒(IBDV)多聚蛋白DNA疫苗分别免疫SPF鸡,14日龄时首免,2周后二免,二免后5周用IBDV标准强毒株BC6/85攻击.研究发现,鸡IL-18能显著促进T、B淋巴细胞的增殖反应,增强IBDV多聚蛋白DNA疫苗诱导的中和抗体水平及其对强毒攻击的保护力.结果提示,鸡IL-18(200μg)对IBDV多聚蛋白DNA疫苗具有显著的免疫增强作用(P<0.05).

To furt her evaluate the immunoenhancement of chicken interleukin- 18 （IL-18）, the polyprotein of infectious bursal disease virus （IBDV） was prepared to DNA vaccine, and the DNA vaccine was coadministrated with the eukaryotic expression plasmid encoding interleukin-18 （pCI-IL-18）, then boosted after 2 weeks, and challenged with virulent IBDV 5 weeks later. It is observed that chicken IL-18 could significantly enhance the proliferation responses of T lymphocytes from spleen and B lymphocytes from bursa. Meanwhile, it can raise the neutralization antibody level and enhance the protection against virulent IBDV induced by DNA vaccine. The results indicate the strong enhancement of immune responses to IBDV DNA vaccine by co-administration with chicken IL-18 （200/μg） （P〈0.05）.