环氧化酶抑制剂Celecoxib增强脑胶质瘤SHG44细胞辐射敏感性的体外实验研究

Enhancement of human glioma cells SHG44 radiosensitivity with celecoxib in vitro studies

为了研究COX-2选择性抑制剂celecoxib对人脑胶质瘤细胞SHG44的辐射增敏作用,用MTT法检测celecoxib对细胞存活分数的影响,用克隆法、RT-PCR法检测celecoxib或联合60Coγ射线照射与细胞克隆存活率及COX-2 mRNA表达水平的关系,探讨celecoxib辐射增敏的可能作用机制,为临床有效治疗胶质瘤提供实验依据。结果表明celecoxib细胞毒性作用随浓度升高而升高;celecoxib能抑制细胞克隆形成,联合60Coγ射线照射显示出协同作用;与对照组、单独药物和照射组相比较,celecoxib联合照射后COX-2 mRNA的表达水平均有所降低。本实验研究认为COX-2选择性抑制剂celecoxib对人脑胶质瘤SHG44细胞具有辐射增敏作用,其机制与COX-2 mRNA表达水平密切相关。

In order to understand the radiosensitizing effects on human glioma cells SHG44 using celecoxib, a cyclooxygenase （COX）-2 selective inhibitor, MTT assay was used to determine the effect of celecoxib on the cell growth, and Colony Formation assay. Reverse transcription-PCR assay were used to investigate the effect of celecoxib or combined with ^60Coγ-irradiation on cell colony formation rate and the levels of COX-2 mRNA expresstion .Experimental results suggested that the cytotoxicity of celecoxib enhanced along with the increment of drug＇s concentration. The celecoxib could inhibit colony formation in SHG44 cells. When combined with ^60Coγ-irradiation, COX-2 mRNA expresstion levels was lower than that of control, drug and irradiation group respectively. The study confirmed the radiosensitizing effects of this drug to human glioma cells SHG44, and it might be closely related to the COX-2 mRNA expresstion levels.