尿源性大肠埃希菌产超广谱β-内酰胺酶的检测及耐药监测

Detection of ESBLs Produced by E. coli in Urinary Tract and Monitoring of Drug Resistance of ESBLs-producing E.coli

目的:了解本地区泌尿道分离大肠埃希菌产超广谱β-内酰胺酶(ESBLs)情况及耐药现状。方法:采用法国梅里埃ATB-Expression分析仪进行细菌鉴定,用K-B法进行药敏试验,并用纸片确证试验检测ESBLs。结果:产ESBLs大肠埃希菌检出率为31.8%,107株大肠埃希菌对亚胺培南100%敏感,对其余抗生素出现不同程度的耐药,产ESBLs株耐药率明显高于非产ESBLs株。结论:产ESBLs大肠埃希菌耐药严重,应重视ESBLs的检测,根据药敏试验结果合理选用抗生素。

OBJECTIVE：To investigate the condition of ESBLs produced by E .coli isolated from urinary tract and the drug resistance of ESBLs- producing E.coli.METHODS： The identification of bacteria was performed using ATB- Expression analysator（France） ; the susceptibility test was performed using K-B method, and ESBLs were detected using disc diffusion confirmatory test.RESULTS： The detection rate of ESBLs- producing E.coli was 31.8% .All（100%） of the 107 strains of ESBLs- producing E. coli were sensitive to imipenem, however,in which different degree of resistance to other antibiotics was noted .The resistance rate was significantly higher in ESBLs- producing strains than in non- ESBLs- producing strains .CONCLUSION： In view of the high antibiotic resistance of ESBLs-producing E.coli, great importance should be attached to the detection of the ESBLs .Antibiotics should be used rationally based on the results of susceptibility test.