家兔魏氏梭菌的分离与半套式PCR鉴定

The isolation and half-nested PCR identification of Rabbits Clostridium perfringens

魏氏梭菌病是危害养兔业的重要疾病。某养兔场发生一种以急性黑色水样腹泻或排带血的胶冻样粪便、盲肠出血、胃粘膜出血为主要特征的传染病,通过病原菌的分离培养、形态染色观察、生化鉴定和半套式PCR反应,分离出一株魏氏梭菌。通过药物敏感实验筛选出了敏感药物。实验结果为该病的正确防控措施的提出提供了参考。

Rabbit clostridial disease is an important disease that threat rabbit production. An acute rabbit infectious disease characteristic of dark diarrhea or gumLike blood feces and cecum bleeding and stomach mucosa bleeding occurrencd in a rabbit farm. A Clostridium perfringens strain was isolated through pathogen isolation, morphologic observation, biochemical reaction and half-nested PCR reaction. Sensive drug were selected by drug sensive experiment. This experiment resuLt provide reference for the establishment of precaution measure of this disease.