巨大芽胞杆菌环氧水解酶的固定化及其催化性能

Immobilization of Bacillus megaterium Epoxide Hydrolase and Its Catalytic Performance

考察了多种载体对巨大芽孢杆菌ECU1001环氧水解酶的固定化.以大孔DEAE-纤维素离子交换树脂为载体时,固定化酶的活力回收达70%.进一步考察了温度和pH对固定化酶活力的影响,并使用该固定化酶进行了缩水甘油苯基醚对映选择性水解批次反应.结果表明,在较低的底物浓度下该固定化酶的稳定性较好,10批反应后仍然剩余72.4%的活力.

The immobilization of Bacillus megaterium ECU1001 epoxide hydrolase was investigated. Macroreticular DEAE-cellulose was selected to immobilize the epoxide hydrolase and the activity retention was about 70%. The effect of temperature and pH on the immobilized epoxide hydrolase was measured. Batch reactions of the hydrolysis of rac-glycidyl phenyl ether were conducted with the immobilized epoxide hydrolase at a low substrate concentration. The residual enzyme activity of 72.4 % was achieved after 10 cycles of reaction.