固体培养基分离解脲支原体和人型支原体探讨

Isolation and Culture of Ureaplasma urealyticum and Micoplasma hominis by Solid Medium

目的:研究固体培养基用于临床检测解脲(Uu)和人型支原体(Mh)及其培养的方法,方法:在原有A7固体培养基配方基础上增加营养物质和抑菌剂,制备新的固体培养基。对46例泌尿生殖道棉拭子直接用固体培养分离Uu和Mh,同时接种液体培养基作为对照。观察并记录16h、24h、40h、48h以后固体培养基长出支原体菌落的阳性例数和液体培养阳性例数,以及两种方法的杂菌污染例数,并用统计学方法分析两种方法的差异。结果:固体培养基直接分离培养结果与液体培养在不同时间段所得到的结果,阳性率无统计学差异,94.4%的菌落于24h～40h之间可辨认。直接划液体培养基的杂菌污染率分别为10.87%、28.2%和10.87%。前两者统计学有差异。结论:改进的固体培养基直接分离Uu和Mh具有鉴别准确,选择性强和菌落出现早且特征明显的优点,与液体培养相比更适用于支原体的临床检测。

Objective To improve traditional method for detecting Mycoplasma with broth media alone,and to research a new solid medium for isolating and identification Methods An attempt was made to modify A7 agar medium by adding mutritional component and other bacteria inhibitors. 46 genital swabs were carefully cultured on the agar media and inoculated in the urea broth and arginine broth.The positive numbers and contaminated numbers with culture time of 16 h, 24h,40h,48h and over-48h were observed and recorded.Results 94.4% of colonies were identified from 24h to 40h.The differences in the positive the turbidity rate was statistically significant （P〈0.05）.Conclusion Having advantages of early accurate detection and bacteria exclusion,the modified new solid media is more applicable to clinical detection for mycoplasma compared with broth media.