人Neurturin基因的克隆及序列分析

Cloning and sequence analysis of human Neurturin

文章以人胎脑cDNA为模板,采用巢式PCR方法扩增人Neurturin成熟蛋白基因,并将其插入到质粒pGEM-T,构建克隆载体pGEM-T-hNTN。对阳性重组子进行鉴定和序列测定,并与已克隆的人Artemin成熟蛋白基因进行同源性分析。结果表明,所克隆DNA片断与文献发表序列(GenBank NM004558)完全一致,人Neurturin成熟蛋白基因与Artemin同源性为58.5%。

With cDNA of human fetal brain as the template, the matured human Neurturin gene was amplified by nested PCR and inserted into the plasmid pGEM-T to construct the cloning vector pGEM-T-hNTN. The positive recombinant was identified and sequenced, and its homology of gene and protein with the matured human A rtemin was analyzed. The results showed the sequence of the cloned DNA fragment was the same as the reported one （GenBank NM004558） completely, and the homology between Neurturin and Artemin was 58.5%.