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传染性支气管炎病毒(IBV)小囊膜蛋白基因的原核表达 被引量：4

Prokaryotic expression of small envelope protein gene in avian infectious bronchitis virus (IBV)
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摘要 以传染性支气管炎病毒(IBV)M41毒株为材料,克隆该毒株的小囊膜蛋白(E)基因,并将其连接到表达载体pGEX-6P-1中,转化到大肠杆菌BL21中。经IPTG诱导后,SDS-PAGE分析目的蛋白与谷胱苷肽硫转移酶(GST,大小约为26ku)融合后大小约为38ku,表明目的蛋白相对分子质量约为12.4。这一结论为E蛋白的进一步研究提供了条件。 0The small envelope protein （E） gene of avian infectious bronchitis virus （IBV） M41 strain was cloned, then subcloned into prokaryotic expressing vector pGEX-6P-1. The recombinant plasmid was transformed into E.coli. BL21 and induced by IPTG. SDS-PAGE demonstrated that when objective protein fused with GST formation to the subsequent E protein research.
作者 张芳 曾祥伟 明晓波 魏萍 祝玉卿 王琳
机构地区 东北农业大学动物医学学院
出处 《东北农业大学学报》 CAS CSCD 2008年第1期99-103,共5页 Journal of Northeast Agricultural University
基金 黑龙江省教育厅科学技术研究项目SARS专项资金(2003fz012)
关键词 传染性支气管炎病毒(IBV) 小囊膜蛋白(E) 蛋白表达 avian intectious bronchitis virus （IISV） small envelope protein （E） protein expression
分类号 Q786 [生物学—分子生物学]
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参考文献6

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同被引文献36

  • 1周玉长,刘胜旺,孔宪刚.鸡传染性支气管炎病毒E蛋白基因的表达[J].中国病毒学,2006,21(1):78-80. 被引量：2
  • 2萨姆布鲁克J,费里奇E F,曼尼阿蒂斯T著.分子克隆试验指南[M].北京:科学出版社,2002:822-897.
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  • 8Das S,Maitra U.Mutational analysis of mammalian translation initiation factor 5(e IF5):Role of interaction between the beta subunit of e IF2 and e IF5 in e IF5 function in vitro and in vivo[J].Mol Cell Biol,2000,20(11):3942-3950.
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东北农业大学学报
2008年 第1期

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