摘要
在含多菌灵药物培养基上，采用紫外线重复诱导处理的方法，对拮抗性木霉T21进行改良，筛选到5株在多菌灵2000mg／L浓度下仍能较好生长的具有显著抗性菌株T21—1。T21—5，通过抗药遗传稳定性和对灰霉菌拮抗性测定，从中选育出性能较优良的抗药性菌株T21．4，并与多菌灵协同作用进行防治黄瓜灰霉病试验。结果表明：菌和药配比在9：1，8：2，7：3时协同防治效果分别达到75．7％，78．9％和79．5％；单独使用T21．4和多菌灵的防效分别为70．5％和71．7％。表明菌和药协同作用防治效果明显高于单独使用的防效。因此，在使用木霉T21—4菌剂防治农作物病害时，添加少量的多菌灵，可降低植物病原菌的活性和侵染力，提高生物菌剂生防效果。
Five carbendazim resistance strains T21-1 - T21-5 were induced by UV-light and selected from Trichoderma spp., which could grow well in the medium, concentration Carbendazim below 2000mg/L. Through a testing of genetic stability of resistance and antagonism of Botrytis cinerea, an ideal resistant strain T21-4 was screened, with a Co-interaction. the combination of T21-4 agent and Carbendazim was used to control Botrytis cinerea of cucumber, the results showed that the control rote reaches 75.7%, 78.9% and 79.5% by treatment of T21-4 and Carbendazim at the ratio of 9： 1, 8：2 and 7：3, respectively. The control rate is only reaches 70.5% and 71.7%, respectively by applving T21-4 or Carbendazim alone. Adding little Carbendazim to Trichoderma T24 to control crops disease, not only reduceds the activity and infection ability of plant pathogens, but also improved the biocontrol efficiency.
出处
《核农学报》
CAS
CSCD
北大核心
2008年第1期32-35,共4页
Journal of Nuclear Agricultural Sciences
基金
河北省科技攻关项目(052413D)
关键词
紫外线诱导
抗药性木霉
抗药性
灰霉菌
UV-induction
resistant Trichoderma
resistance
Botrytis cinerea
