紫菜薹快速碱化因子基因BcMF14p的序列与表达分析

MOLECULAR CHARACTERIZATION AND EXPRESSION PATTERN OF RALF-LIKE GENE BcMF14p FROM Brassica campestris var.purpurea

快速碱化因子是近年来新发现的一种植物多肽类信号分子。根据已知的普通白菜两用系中可育株系特异表达的快速碱化因子基因BcMF14序列,在其编码框两侧设计引物,从紫菜薹中克隆出该类信号分子基因,命名为BcMF14p(登陆号:EF523517),全长273 bp,不含内含子,编码框包含222个核苷酸,编码73个氨基酸。NCBI在线同源比对结果表明该基因属于快速碱化因子家族,与花椰菜、拟南芥等的快速碱化因子核苷酸序列相似性达到86%以上。对该信号分子的表达进行RT-PCR分析,结果显示BcMF14p只在紫菜薹花蕾、花、角果等生殖器管中表达,在茎、叶等营养器官中不表达,说明BcMF14p可能参与紫菜薹生殖发育过程中的信号传导。

The rapid alkalinization factor （RALF） gene is one of polypeptide signals. In this study BcMF14p was cloned from Brassica campestris ssp. chinensis var. purpurea based on BcMF14 from B. campestris ssp. chinensis var. communis cv. Aijiaohuang. The sequence of this gene was 273bp （GenBank accession number EF523517）, including a 222 bp ORF, colding 73 amino acids, It considered as RALF-like protein according to its high identity with B. oleracea var. botrytis BoRALF1 and Arabidopsis thaliana RALF-8 in nucleotide sequence （ 〉 86% ）. RT-PCR discovered that BcMF14p was expressed prominently in flower buds, flower and silique, but no expression in vegetative organs, this indicated that BcMF 14p may be involved in the signal transduction during reproduction development of B. campestris var. purpurea.