鲍曼不动杆菌整合子相关耐药基因研究

Studies on multidrug-resistance related with integrons in Acinetobacter baumannii

目的了解我院鲍曼不动杆菌整合子流行情况,证实整合子与鲍曼不动杆菌多重耐药性的关系,建立一种快速简便的整合酶聚合酶链反应(PCR)检测方法。方法收集我院2005年9月至2006年2月临床分离的鲍曼不动杆菌共52株,用纸片扩散法检测鲍曼不动杆菌耐药性及用整合酶PCR检测其整合子基因。结果在52株鲍曼不动杆菌中,整合酶PCR检测出Ⅰ类整合子33株(63%),未检测出Ⅱ类整合子。统计学分析结果显示,整合子阴性和阳性的鲍曼不动杆菌对各种抗生素的耐药率存在明显差异,前者仅对几种抗生素耐药而后者对多种抗生素耐药。结论我院多重耐药鲍曼不动杆菌中主要为Ⅰ类整合子,整合酶PCR是一种快速、简便、易在临床开展的检测方法,同时也是控制医院感染的有效检测手段。

Objective To investigate the epidemiology of integrons in Acinetobacter baumannii （A. baumannii） from Renji hospital. To confirm the relationship between integrons and muhidrug-resistant A. baumannii. To find out a rapid and simple polymerase chain reaction（PCR） for integrons detection. Methods 52 A. baumannii isolates was collected from September 2005 to February 2006 in Renji hospital. Its antimicirobial susceptibility was determined by K-B method and the integrons gene was detected by integrase PCR. Results Among the 52 A. baumannii isolates,33 integron-positive isolates（63% ） were found by integrase PCR. Class Ⅱ integrons was not detected. Integrons carriage had statistical significance associated with an increase in antibiotic resistance, compared to integrons-negative isolates. Conculsions Muhidrug-resistant A. baumannii mainly carrys class Ⅰ integrons. Integrase PCR is a rapid and simple tool for routine clinical microbiology laboratories detection and is an effective method for the nosocomial infections control.