原位肝移植大鼠肝内门-体分流量的变化

Intrahepatic portal systemic shunting in rats after orthotopic liver transplantation

目的测定原位肝移植大鼠移植肝肝内门-体分流量的变化。方法健康清洁级封闭群雄性SD大鼠,分为供体组和受体组,采用简化二袖套法建立大鼠原位肝移植模型(rat orthotopic liver transplantation model,ROLTM)24只,分为移植后2h、4d、7d三组,每组8只。各组于相应时间点进行肝脏活体在体灌注。经小隐静脉插管连续灌注D-山梨醇,同时取门静脉和肝静脉血,用酶分光光度法测定山梨醇浓度,计算肝内门-体分流量。结果2h、4d、7d组肝内门-体分流量分别为39.9%,52.3%,27.6%,组间差异显著,且均比正常大鼠明显增高。结论ROLTM大鼠移植肝血供恢复后即存在肝内门-体分流,并随时间推移分流量变化呈先增加后减少的过程。

Objective To determine changes in intrahepatic portal systemic shunting （IPSS）. Methods A rat model of orthotopic liver transplantation （ROLTM） was established by using two-cuff technique,and 48 male SD rats were divided into donor-group and acceptor-group. Rats were divided into 3 groups with each group having 8 rats. Rats in group Ⅰ, Ⅱ and Ⅲ received liver reperfusion 2 hours ,4 days and 7 days after successful transplantation,respectively. Liver perfusion was performed in vivo through saphenous vein. Intrahepatic portal systemic shunting was determined by calculating the concentration of D-sorbitol in the venous blood samples by using enzymatic spectrophotometric method. Results Intrahepatic portal systemic shunting in 2 h,4 d and 7 d group were 39.9% ,52.3% and 27.6% ,respectively. Significant differences were found between above 3 groups in intrahepatic shunting that was much higher than that in normal rats. Conclusion Intrahepatic portal systemic shunting in transplanted livers occurred immediately after recovery of blood-supply, and presented changes where intrahepatic shunting increased in early stage but decreased later on.