脂蛋白脂酶基因PvuⅡ酶切位点多态性与胰岛素抵抗关系的研究

DNA polymorphism of pvuII sites in the lipoprotein lipase gene in patients with insulin resistance

目的旨在探讨脂蛋白脂酶基因第6内含子PvuⅡ酶切位点多态性与胰岛素抵抗、2型糖尿病的发生之间是否有关联。方法随机选取163例2型糖尿病患者作为病例组,106例正常人作为正常对照组,对第6号内含子PvuⅡ酶切位点进行多态性分析。结果病例组中P+P+组高密度脂蛋白胆固醇水平明显低于P-P-组,而血清三酰甘油、胰岛素抵抗指数指标水平高于P-P-组。结论PvuⅡ酶切位点多态性与2型糖尿病中胰岛素抵抗有关。

Objective To investigate the frequency of variant of lipoprotein lipase（LPL）gene,PvuⅡ at intron 6 ,and its relation to insulin resistance（IR） in type 2 diabetes mellitus. Methods PCR-RFLP method was used to determine the DNA polymorphism of 6th intron at LPL gene in 163 type 2 diabetes（T2DM）patients and 106 controls. Results The levels of TG and IR in P ＋ P ＋ genotype were higher than those in P-P-genotype （ P 〈0.05） ,but HDL-C was lower（P 〈 0.05）. Conclusion The LPL-PvuⅡ-RFLP is significantly associated with insulin resistance in type 2 diabetes.