环氧氯丙烷对戊二醛鞣制的猪主动脉瓣防钙化化学改性的实验研究

Research on the modified anti-calcification feature of glutaraldehyde crosslinked porcine aortic valv by epoxy chloropropane treatment

目的探索对戊二醛鞣制猪主动脉瓣改性的新的防钙化处理方法,延长生物瓣使用寿命。方法用环氧氯丙烷对戊二醛鞣制的猪主动脉瓣进行改性处理,新鲜瓣膜组作为空白组,戊二醛处理组作为对照组。将三组猪主动脉瓣埋植于大鼠皮下,分别于2、4、8周后取出,通过原子吸收光谱法测定猪主动脉瓣钙含量和VonKossa钙染色,以评价环氧氯丙烷化学改性对戊二醛鞣制的猪主动脉瓣钙化的影响。结果光镜下显示环氧氯丙烷改性处理组较少炎性细胞浸润,胶原纤维结构保持良好;戊二醛组试片,则有大片状钙盐沉积及较多炎性细胞浸润,胶原纤维结构破坏严重;SD大鼠皮下包埋实验各组瓣膜的钙化程度在第2、4、8周时逐渐增加,在第4、8周时实验组明显低于对照、空白组(P<0.05)。说明环氧氯丙烷对戊二醛鞣制的猪主动脉瓣改性处理,可显著延缓猪主动脉瓣的钙化进程。Von Kossa染色也显示环氧氯丙烷改性处理明显减轻了戊二醛鞣质的生物瓣膜的钙化。结论通过环氧氯丙烷对猪主动脉生物瓣进行进一步的化学改性处理,可显著延缓猪主动脉生物瓣的钙化进程,可望成为一种防止猪主动脉生物瓣钙化和坏死及其功能衰退的有效方法。

Objective To study for a new chemical treatment that reduces the calcification and improves the durability of glutaraldehyde cross-linked porcine aortic valv. Methods Fresh porcine aortic valv was cross-linked with glutaraldehyde,followed by chemical treatment with epoxy chloropropane. Then implanted subcutaneously into the backs of SD rats for 2,4 and 8 weeks. Calcium content was determined by atomic absorption spectrometry and Von Kossa staining. Results At 8 weeks after implantation ,the calcium content of epoxy chloropropane groups were significantly lower than that in glutarldehyde and fresh valve group;Von Kossa staining also appeared to be less calcified. Conclusion The HE staining confirmed that the epoxy chloropropane could keep the integrality of conllagen. The glutaraldehyde cross-linked porcine aortic valv treated with epoxy chloropropane appeared to be less calcified.