血小板1bα基因多态性与不稳定性心绞痛关系的研究

Relationship between polymorphism of platelet membrane glycoprotein 1bα gene and unstable angina pectoris disease

目的为了探讨血小板1bα基因多态性与中国人群不稳定性心绞痛的关系。方法随机收集90例不稳定性心绞痛患者,并按年龄、性别相匹配收集90例健康对照人群,观察不稳定性心绞痛与血小板糖蛋白1bα145Thr/Met变异的关系。采用聚合酶链反应-限制性片段多态性(PCR-RFLP)方法进行血小板糖蛋白1bα145Thr/Met变异的分析。结果不稳定性心绞痛组血小板GP1bα基因145M/M纯合子为2例,145T/M杂合子为16例,突变率20.00%,对照组纯合子为0例,杂合子为11例,突变率12.22%,结果显示两组血小板糖蛋白1bα145Thr/Met差别有显著意义(P=0.004)。OR=2.12,95%CI:1.13～3.18。排除高血压、糖尿病、高血脂、吸烟史、冠心病家族史等因素,受试者UA的发生率与血小板膜GPⅠbα基因145Thr/Met变异仍然有相关性(OR=1.001,95%CI:0.7～1.8)。结论血小板糖蛋白1bα145Thr/Met变异与中国人群不稳定性心绞痛的发生相关。

Objective To explore the relationship between polymorphism of platelet membrane glycoprotein 1 bα gene and unstable angina pectoris. Methods The study consisted of 90 unstable angina pectoris, and an age and sex matched control per case, the controls were selected from healthy people. The study observed difference genotype frequencies of dimorphism of the 145Thr/Met in the platelet membrane glycoprotein 1bα gene of the unstable angina pectoris and healthy people. PCR-RFLP was used to analyze polymorphism of the 145Thr/Met in the platelet membrane glycoprotein 1bα gene. Results 145Thr/Met in the platelet membrane glycoprotein 1bα gene was found in 18 of 90 patients with unstable angina pectoris（ 20.00% ）and in 11 of 90 healthy control subjects（ 12. 22% ）. This difference was statistically significant （P = 0. 004）. OR = 2. 12,95% CI：1. 13-3. 18. Conclusion The present study demonstrates that 145Thr/Met in the platelet membrane glycoprotein 1bα gene is closely related to the unstable angina pectoris, suggesting that this 145Thr/Met is an inherited risk factor for the unstable angina pectoris.