银杏叶片中总黄酮醇苷的含量测定

Content determination of total favonol glycosideds in ginkgo tablet

目的建立反相高效液相色谱法测定银杏叶片样品中总黄酮醇苷（槲皮素、山柰素、异鼠李素）的含量测定方法。方法采用phenomenex C18色谱柱，流动相为甲醇-0．4％磷酸水溶液（50：50），流速为1．0ml／min，检测波长为360nm，柱温：25℃。结果总黄酮醇苷中槲皮素在与山柰素在0．0075～0．06mg／ml（r=0．9999，n=5）、异鼠李素在0．005～0．04mg／ml（r=0．9995，n=5）浓度范围内具有较好的线性关系；平均加样回收率槲皮素为99．92％（RSD=1．65％，n=6）。山柰素为98．60％（RSD=1．3％，n=6），异鼠李素为99．41％（RSD=0．81％，n=6）。结论该法操作简便、灵敏度高、重现性好，可用于银杏叶片中总黄酮醇苷的含量测定。

Objective To establish a HPLC method for determination the content of total favonol glycosides in ginkgo tablet. Methods Phenomenex C18 column was used as chromatographic column,and methanol-0.4% acid water（50：50） as mobile phase. The detection wavelength was set at 360 mm, flow rate at 1.0 ml/min,and column temperature at 25℃. Results There was a good linear relationship when quercetin and kaempferol of total favonol glycosides were in the range of 0. 007 5 - 0.06 mg/ml （ r = 0. 999 9, n = 5 ） , and Isorhamnetin in the range of 0. 005 - 0. 04 mg/ml （ r = 0. 999 5, n = 5 ）. The average recoveries of Quercetin, kaempferol and isorhamnetin were respectively 99.92% （ RSD = 1.65% , n = 6） ,98.60% （ RSD = 1.3% , n = 6） ,99.41% （RSD =0.81% ,n =6）. Conclusion This method is simple in operation,high in sensitivity and good in reproducibility,and can be used for the determination of the preparation.