摘要
目的探讨培养法检测肺炎支原体(MP)的临床应用价值。了解该地区儿童MP的感染情况。方法取患者咽拭子样本接种于MP选择鉴别培养基进行培养,并将检测结果与酶免法、PCR法检测结果进行比较分析。结果培养法检测945份样本,阳性率为37.99%(359/945);ELISA法检测270例,MP-IgM阳性率为13.3%(36/270)。荧光定量PCR法与培养法阳性符合率为100%。结论培养法检测MP阳性明显高于ELISA法,可作为早期诊断的金标准,且方法简便快速,易于推广。
Objective To evaluate the value of culture detection for mycoplasma pneumoniae in clinical application. Methods Mycopasma culture was used to detect Mp in throat samples were collected on cotton-tipped swabs,and compared the results of ELISA and PCR and culture. Results The 945 throat samples were detected by mycoplasma culture and the positive rate was 37.99% （359/945）. The 270 blood samples were detected by ELISA and the positive rate was 13.3% （36/270）. The results of PCR and culture was consistent. Conclusion The positive rate of mycoplasma culture was significantly higher than that of PCR,and it can be the golden standard in the diagnosis of mycoplasma pneumoniae.
出处
《中国实用医药》
2008年第6期34-35,共2页
China Practical Medicine
关键词
肺炎支原体
培养
诊断
Mycoplasma pneumoniae
Culture
Diagnosis
