反硝化细菌对硫酸盐还原菌的竞争抑制研究

Study on the competitive inhibition of denitrifying bacteria with sulfate reducing bacteria

以从大庆油田采出水中筛选到的硫酸盐还原菌SRB-2和反硝化细菌DNB-1为目的菌，考察两者之间的竞争抑制关系，以及添加营养物质的种类和浓度对SRB-2数量及产H2S活性的影响。结果表明，当体系中硝酸盐浓度为0.5 g/L或亚硝酸盐浓度为0.1-0.5 g/L时，DNB-1就能够有效降低SRB-2活性，抑制H2S的产生，抑制时间10 d以上，H2S产生可以减少85%以上；当体系中硝酸盐浓度为0.1-0.25 g/L时，在10 d内DNB-1对SRB-2活性和产H2S活性也有较好的抑制效果，H2S产生减少65%左右；亚硝酸盐对SRB-2生长的抑制作用好于硝酸盐。

DNB-1 and SRB-2 isolated from flooding water in Daqing oil field were used to study the competitive inhibition relations between the two bacteria species and the effects of nitrate and nitrite amendment. The results showed that H2S production was decreased effectively with nitrate amendment 0.5 g/L or nitrite 0. 1 - 1.0 g/L, indicating that DNB-1 can competitively inhibit SRB-2 activity. The inhibition effect was retained for more than 10 days,and 85% H2S production was decreased; DNB-1 can inhibit SRB-2 activity and H2S production within 10 days with 0. 1 - 0.25 g/L nitrate amendment and 65% H2S production was decreased. The inhibition effect of nitrite was better than that of nitrate.