脑脉通对老龄大鼠脑缺血/再灌注明胶酶系基因表达调节作用的影响

Effects of Naomaitong on regulateing gene expression of gelatinase system after cerebal ischemia/reperfusion in aged rats

目的:研究脑脉通对大鼠脑缺血/再灌注(I/R)不同时期微血管基底膜损伤和明胶酶系基因表达作用的影响。方法:制备局灶性脑I/R模型,将大鼠分为假手术组、模型组、脑脉通组、尼莫地平组,后3组又各分为脑缺血3h和I/R 6h、12h、24h、3d、6d时间点组。采用透射电镜、原位杂交方法,观察各组微血管结构、明胶酶及其抑制物的基因表达变化。结果:药物组电镜下其病理损伤较轻。明胶酶系基因表达显示,脑脉通可降低模型组(I/R 6h-6d)MMP-2 mRNA、(I/R 6h、I/R 12h、I/R 3d)MMP-9 mRNA的表达,提高模型组(I/R6h-3d)TIMP-1m RNA的表达。结论:脑脉通对大鼠脑I/R微血管基底膜损伤的保护作用与其对明胶酶系基因表达调节有关。

Objective： To study effects of Naomaitong on injury of cerebro-microvessel basement membrane in different periods and gene expression of gelatinase system after cerebral ischemia/reperfusion （I/R） in rats. Methods： Focal cerebral ischemia/reperfusion model was established by MCAO method of the intraluminal filament technique. Rats were divided into sham-operated group, model group, Naomaitong group and Nimodipine group at random, the later three groups with ischemia 3h and I/R 6h, 12h, 24h, 3d, 6d. Ransmission electron microscope and hybridization in situ method were used to observe the structure of micrangium and gene expression of gelatinase and its inhibitor. Results： Under electron microscope, the patho-damage in medico-group seemed lighter. According to the gene expression of gelatinase system, Naomaitong could step down the mRNA expression of M MP-2（I/R 6h- 6d）and MMP-9（I/R 12h, I/R 3d）. And increase the mRNA expression of TIMP-I （I/R 6h- 3d）. Conclusion： The protection of Naomaitong on cerebro-microvessel basement membrane after cerebral I/R in rats are related to the gene expression regulation of gelatinase system.