睿祺片对胎鼠大脑皮层神经元内钙离子水平的影响

Effect of Ruiqi pill on neuronal calcium level of cerebral cortex in brain of rat embryos

目的:探讨睿祺片对胎鼠大脑皮层神经元内游离钙离子水平的影响。方法:取妊娠16-17d Wistar大鼠的胚胎大脑皮层,培养4-5d随机分为睿祺片组及空白对照组,应用激光共聚焦扫描显微镜测定胎鼠大脑皮层神经元原代培养在加入睿祺片水溶液后细胞内钙离子水平的变化。结果:睿祺片组可降低神经细胞内游离钙离子浓度,空白对照组无此变化。结论:睿祺片可降低神经细胞内游离钙离子浓度,这可能是其改善记忆的作用机制之一。

To explore the effect of Ruiqi pill on neuronal calcium level of cerebral cortex in brain of rat embryos. Methods： The cerebral cortex of rat embryos pregnanted for 16-17 days were dissected. The neural cells were divided into Ruiqi pill group and control group randomly after 4-5 days in vitro culture. Effect of Ruiqi pill on neuronal calcium level of primary culture of cortical neurons in rat embryos was measured by laser scanning confocal microscopy. Results： Ruiqi pill could reduce neuronal calcium concentration, no change was found in the control group. Conclusion： Ruiqi pill reduced neuronal calcium concentration, which might be one of the mechanisms of Ruiqi pill in improving memory function.