正交试验法优选白头翁醇提工艺

Optimization of alcohol-extraction technology for Radix Pulsatillae by orthogonal design

目的:优选白头翁的最佳醇提工艺。方法:以测定白头翁中皂苷B4提取率作为考察指标,采用L9(34)正交试验,考察乙醇浓度、浸提时间、浸提次数对白头翁皂苷提取的影响。结果:白头翁的最佳醇提工艺为75%乙醇,提取3次,浸提时间3h。结论:本工艺可为提取白头翁生产工艺作参考。

Objective： To optimize the alcohol-extraction process and condition of Radix Pulsatillae. Methods： Alcohol concentration, the extraction time and number of extraction on effect of extraction rate of pulehinenoside B4 were evaluated by orthogonal design L9 （3^4）. Results： The optimum condition of the alcohol extraction was： 75% ethanol, 3 times of extraction, 3h for extraction process. Conclusion： The extraction method can be used for reference for the extraction process of Radix Pulsatillae.