鲜猪肉小肠结肠炎耶尔森氏菌的分离鉴定

ISOLATION AND IDENTIFICATION OF YERSINIA ENTEROCOLITICA FROM FRESH PORK

对重庆市２１５份鲜猪肉污染小肠结肠炎耶尔森氏菌分离鉴定和增菌、分离培养基选择结果表明，猪肉中小肠结肠炎耶氏菌污染率为２．７９％，６株分离菌均为生物３型。其中，５株属０∶３血清型，１株为０∶６．３０血清型。在２５℃时，用１．５％去氧胆酸钠增菌效果显著。分离培养基以ＳＳ，ＭＴ，ＳＳ－Ｄ和ＳＳ－Ｍ琼脂培养基较好。

Yersinia enterocolitica was isolated and identified from 215 fresh pork samples collected from 5 districts and counties of Chongqing, and suitable enrichment media and isolation media for it were selected. Its contamination rate in the fresh pork averaged2.79%. Altogether six strains of the bacterium were isolated and biochemical determination showed them all to be of biotype 3. When subjected to slide agglutination experiment, five of them were shown to be of 0∶3 serotype and the other one of 0∶6.30 serotype. Cultured at 25℃, the addition of 1.5% sodium beoxycholate gave satisfactory enrichment result. Of the isolation media studied, SS, MT, SS D and SS M appeared to be desirable.