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甜菜坏死黄脉病毒(IBNYVV)外壳蛋白分子量的测定 被引量：1

Detection of Molecular Weight of Coat Prtein of Bnyvv
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摘要 通过对甜菜坏死黄脉病毒提纯液进行紫外扫描，获得了典型的核蛋白曲线，ＯＤ２８０／ＯＤ２６０＝０．９０１，推算病毒液浓度约为０．５８ｍｇ／ｍｌ。采用ＳＤＳ－ＰＡＧＥ测定证实ＢＮＹＶＶ只有１种蛋白亚基，分子量为２０７４９道尔顿。 Through ultra-violet Scanning of the Purified Solution of BNYVV a typical nucleo-protein curve,OD 280/OD260=0.901 was obtained and the concentration of the virus solution was calculated as about 0.589mg/ml It was Proved that through SDS-PAGE detection that BNYVV has only one protein subunit and its molecular weight ys 20749 dalton
作者 张蓉 白生海 蘖翠峰 罗勤
机构地区 宁夏农林科学院植保所 中国科学院武汉病毒研究所
出处 《宁夏农林科技》 1997年第4期10-12,共3页 Journal of Ningxia Agriculture and Forestry Science and Technology
关键词 甜菜 BNYVV 分子量 Sugar beet BNYVV Molecular Weight
分类号 S435.663 [农业科学—农业昆虫与害虫防治]
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参考文献2

  • 1陈小江,龚祖埙,尹玉琦,崔星明,李国英.甜菜坏死黄脉病毒的分离提纯及其生物化学特性的研究[J]石河子农学院学报,1986(01).
  • 2魏宁生,HuthW.雀麦花叶病毒德国株系与英国株系病毒粒子的比较研究[J]植物病理学报,1984(02).

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  • 1Mayer S A,Averhoff P,Zychlinsky A.How do neutrophils and pathogens interact[J].Curr Opin Microbiol,2004,7(1):62-66
  • 2Kogut M H,Tellez G I,McGruder E D,et al.Evaluation of Salmonella eraeritidisimmune lymphokines on host resistance to Salmonella enteritidis ser.Gallinarum in broiler chicks[J].Avian Pathol,1997,25(4):734-747
  • 3Hu Y C.Baculovirus vectors for gene therapy[J].Adv Virus Res,2006,68:287-320
  • 4Luckow V A,Summers M D.Signals important for high-level expression of foreign genes in Autographa californica nuclear polyhedrosis virus expression vectors[J].Virology,1988,167 (1):56-71
  • 5Gronowski A M,Hilbert D M,Sheehan K C F,et al.Baculovirus stimulates antiviral effect in mammalian cells[J].J Virol,1999,73(12):9944-9951
  • 6Abe T,Takahashi H,Hamazaki H,et al.Baculovirus induces an innate immune response and confers protection from lethal influenza virus infection in mice[J].J Immunol,2003,171:1133-1139
  • 7Niu M,Han Y,Li W.Baculovirus up-regulates antiviral systems and induces protection against infectious bronchitis virus challenge in neonatal chicken[J].Int Immunopharmacol,2008,8 (12):1609-1615
  • 8Haiqi H,Kenneth J G,Christina L S,et al.Differential induction of nitric oxide,degranulation,and oxidative burst activities in response to microbial agunist stimulations in monocytes and heterophils from young commercial turkeys[J].Vet Immunol Immunop,2008,123(3-4):177-185
  • 9Wilson S,Baird M,Ward V K.Delivery of vaccine peptides by rapid conjugation to baculovirus particles[J].Vaccine,2008,26 (20):2451-2456
  • 10Michael K,Virginia K L,Morgan F.Selective pharmacological inhibitors reveal the role of Syk tyrosinc kinase,phospholipase C,phosphatidylinositol-3V-kinase,and p38 mitogen-activated protein kinaae in Fc receptor-mediated signaling of chicken heterophil degranulatian[J],Int Immunopharmacol,2002,2(7):963-973

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宁夏农林科技
1997年 第4期

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