摘要
黄酮类化合物因具有多种生物活性而成为天然产物研究的热点。通过直接测定法、硝酸铝法、氯化铝法3种检测方法的比较,确定氯化铝法作为柑桔皮总黄酮的测定方法效果更好,其检测波长为275nm,以芦丁为标准品,在0.0077mg/mL～0.0385mg/mL范围内,其浓度与吸光度呈良好的线性关系,相关系数R^2=1,平均加样回收率为99.3%,RSD=1.84%(n=5),稳定性、精密度和重现性均理想,经测定不脱脂超声处理过的柑桔皮中所得黄酮含量为3.09%。
Flavonoids had been widely studied in natural products because of their several bioactivities to human body. The paper compared three detection methods, which are direct method, aluminum nitrate method and aluminum chloride method, and confirmed that aluminum chloride method had the best result for quantitative determination of total flavonoids in citrus peel. If the determination wavelength was 275 nm, the standard sample was rutin and the concentration of analyte was within the range of 0.0077-0.0385 mg/ml, the results showed a good linear relationship （R2=1） between absorbance and the concentration of analyte, with the correlation coefficient R2 of 1. The average recovery of this method was 99.3% with RSD of 1.84% （n=5）. The stability, precision and repetition of this method were very good. The average total flavonoids content of non-isolating fat and ultrasonic treatment citrus peel was 3.09%. This method is easily operated, accurate and reproducible.
出处
《中国酿造》
CAS
北大核心
2008年第2期69-74,共6页
China Brewing
基金
湖南省“十一五”重大科技专项(2006N1002)
湖南省农业科学院“青年学术带头人培养计划”(20051510)
