MMP-9、LMP-1在鼻NK/T细胞淋巴瘤组织中的表达及意义

The Expression and Significance of Matrix Metalloproteinase-9 and Latent Membrane Protein-1 in Nasal NK/T Cell Lymphoma

目的通过检测鼻NK/T细胞淋巴瘤组织中的基质金属蛋白酶-9(MMP-9)、潜伏膜蛋白-1(LMP-1)蛋白的表达及分布情况,探讨两者间的相互关系,为临床治疗和预后判断提供依据。方法采用免疫组化S-P法检测20例鼻NK/T细胞淋巴瘤组织中MMP-9、LMP-1的表达情况,同时与组织芯片中其他类型淋巴瘤及10例淋巴组织反应性增生进行比较。结果鼻NK/T细胞淋巴瘤组中MMP-9、LMP-1阳性表达率分别为90.0%(18/20)和45.0%(9/20);组织芯片包含的B细胞淋巴瘤组及外周T细胞淋巴瘤组MMP-9的阳性表达率分别为61.1%(11/18)、50.0%(6/12),LMP-1的阳性表达率分别为11.1%(2/18)、25.0%(3/12)。淋巴组织反应性增生组未见LMP-1的阳性表达,MMP-9的阳性表达率为30.0%(3/10)。鼻NK/T细胞淋巴瘤组MMP-9、LMP-1阳性表达率与淋巴组织反应性增生组比较差异有统计学意义(P<0.05),MMP-9与LMP-1表达呈正相关(r=0.327,P<0.05)。结论MMP-9在鼻NK/T细胞淋巴瘤组织中存在高表达,与EB病毒感染关系密切,LMP-1能上调MMP-9表达,可能与鼻NK/T细胞淋巴瘤的高度侵袭性有关。

Objective To detect the expression and distribution of matrix metalloproteinase-9 and latent membrane protein-1 in nasal NK/T cell lymphoma,and to provide evidences for clinical treatment and evaluation of prognosis by investigate the relationship of the both sides. Methods Immunohistochemistry by S-P technique was used to detect the expression of matrix metalloproteinase-9 and latent membrane protein-1 in nasal NK/T cell lymphoma, meanwhile a tissue chip of other type lymphoma and 10 cases of reactive lymphoid hyperplasia were used for relative research. Results The positive expression rate of MMP-9 and LMP-1 in the group of nasal NK/T cell lymphoma was 90.0% （18/20）and 45.0% （9/20）, respectively. Tissue chip consist of the group of B-cell lymphoma and T-cell lymphoma . The positive expression rate of MMP-9 and LMP-1 in the group of B-cell lymphoma was 61. 1%（11/18）and 11. 1% （2/18）, respectively, while those in 12 cases of T-cell lymphoma was 50. 0% （6/12）and 25.0% （3/12）, respectively.There was no positive expression of LMP-1 in reactive lymphoid hyperplasia and the positive expression rate of MMP-9 was 30.0%（3/10）, and the positive expression rates between the group of nasal NK/T cell lymphoma and reactive lymphoid hyperplasia had significant difference statistically（P〈0.05）. The expression of MMP-9 showed a positive correction with the expression of LMP-1（r=0. 327,P〈0.05）. Conclusion MMP-9 is highly expressed in nasal NK/T cell lymphoma which is close correlated with the virus of EB. The induction of MMP-9 by LMP-1 may contribute to the intense invasiveness of nasal NK/T cell lymphoma.